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STEMCELL Technologies Inc human peripheral blood cd14+ monocytes 70035
Human Peripheral Blood Cd14+ Monocytes 70035, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+peripheral+blood+cd14++monocytes+70035/rosettesep+ab+mixture/pm37848444-340-6-13
Average 90 stars, based on 1 article reviews
human peripheral blood cd14+ monocytes 70035 - by Bioz Stars, 2026-10
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Cell Culture:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Recombinant:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Immunofluorescence:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Derivative Assay:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Expressing:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Centrifugation:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Suspension:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Reverse Transcription:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Cell Viability Assay:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

In Situ:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

SYBR Green Assay:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

RNA Sequencing:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Quantitative RT-PCR:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Luciferase:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Software:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Activity Assay:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Fluorescence:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Concentration Assay:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Flow Cytometry:

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: Human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination
Article Snippet: Macrophages were collected by centrifugation and then dissociated into single-cell suspension using trypsin solution (Gibco) at 37 °C for 5 min. For inducing the differentiation of human primary macrophages, human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages
Article Snippet: Human peripheral blood CD14 + monocytes (Stemcell, Cat# 70035) were cultured in ImmunoCult-SF Macrophage Medium (Stemcell, Cat# 10961) containing 50 ng/mL of human recombinant M-CSF (Stemcell, Cat# 10961).

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.
Article Snippet: For inducing the differentiation of human primary macrophages, human peripheral blood CD14+ monocytes (STEMCELL Technologies, 70035) were thawed and cultured in ImmunoCultTM-SF macrophage medium (STEMCELL Technologies, 10961) containing 50 ng/mL of human recombinant M-CSF (STEMCELL Technologies, 78057) for four days.



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a Immunofluorescence images of cells retained after filtering out small blood cells through the ScreenCell® pores. Arrowhead, THCs expressed both macrophage CD86 and epithelial EpCAM markers. Dashed lines, giant macrophage-like THCs. These images are representative of >100 putative THCs analyzed from 10 patients. Scale bar, 50 µm. b – j PBMCs of prostate cancer patients were analyzed by CyTOF ( n = 16 patients). b UMAP depicting different cell types based on their specific markers. CTC, circulating tumor cell; NK, natural killer cell; NKT cell, natural killer T cell; PMN-MDSC, polymorphonuclear myeloid-derived suppressor cell. See also Supplementary Fig. and Supplementary Table . c Pie chart showing the proportions of each cell type identified by CyTOF. d , e The expression levels of mesenchymal markers Vimentin and Fibronectin ( f ) and monocyte/macrophage-related markers CD86, CD163, <t>CD14,</t> and CD16 ( g ) in monocytes/macrophages, THCs, and CTCs. f Left: scatter plot showing the expression levels of CD86 and CD163 in monocytes/macrophages and THCs. Cells were divided into four categories based on the median expression of CD86 and CD163. Right : proportion of the four categories in THCs and monocytes/macrophages. g , h The expression levels of epithelial ( g ) or mesenchymal ( h ) markers of putative THCs in the four categories. i , j Ratios of THC numbers to CTC numbers in individual patients. BCR biochemical recurrence, CR/MET castration resistance and/or metastasis. P -values were determined using a one-way ANOVA followed by Tukey test ( d , e , g , h ) and two-sided unpaired t -test ( i , j ). Source data for i , j are provided as a Source Data file.
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Image Search Results


a Immunofluorescence images of cells retained after filtering out small blood cells through the ScreenCell® pores. Arrowhead, THCs expressed both macrophage CD86 and epithelial EpCAM markers. Dashed lines, giant macrophage-like THCs. These images are representative of >100 putative THCs analyzed from 10 patients. Scale bar, 50 µm. b – j PBMCs of prostate cancer patients were analyzed by CyTOF ( n = 16 patients). b UMAP depicting different cell types based on their specific markers. CTC, circulating tumor cell; NK, natural killer cell; NKT cell, natural killer T cell; PMN-MDSC, polymorphonuclear myeloid-derived suppressor cell. See also Supplementary Fig. and Supplementary Table . c Pie chart showing the proportions of each cell type identified by CyTOF. d , e The expression levels of mesenchymal markers Vimentin and Fibronectin ( f ) and monocyte/macrophage-related markers CD86, CD163, CD14, and CD16 ( g ) in monocytes/macrophages, THCs, and CTCs. f Left: scatter plot showing the expression levels of CD86 and CD163 in monocytes/macrophages and THCs. Cells were divided into four categories based on the median expression of CD86 and CD163. Right : proportion of the four categories in THCs and monocytes/macrophages. g , h The expression levels of epithelial ( g ) or mesenchymal ( h ) markers of putative THCs in the four categories. i , j Ratios of THC numbers to CTC numbers in individual patients. BCR biochemical recurrence, CR/MET castration resistance and/or metastasis. P -values were determined using a one-way ANOVA followed by Tukey test ( d , e , g , h ) and two-sided unpaired t -test ( i , j ). Source data for i , j are provided as a Source Data file.

Journal: Nature Communications

Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination

doi: 10.1038/s41467-023-42303-5

Figure Lengend Snippet: a Immunofluorescence images of cells retained after filtering out small blood cells through the ScreenCell® pores. Arrowhead, THCs expressed both macrophage CD86 and epithelial EpCAM markers. Dashed lines, giant macrophage-like THCs. These images are representative of >100 putative THCs analyzed from 10 patients. Scale bar, 50 µm. b – j PBMCs of prostate cancer patients were analyzed by CyTOF ( n = 16 patients). b UMAP depicting different cell types based on their specific markers. CTC, circulating tumor cell; NK, natural killer cell; NKT cell, natural killer T cell; PMN-MDSC, polymorphonuclear myeloid-derived suppressor cell. See also Supplementary Fig. and Supplementary Table . c Pie chart showing the proportions of each cell type identified by CyTOF. d , e The expression levels of mesenchymal markers Vimentin and Fibronectin ( f ) and monocyte/macrophage-related markers CD86, CD163, CD14, and CD16 ( g ) in monocytes/macrophages, THCs, and CTCs. f Left: scatter plot showing the expression levels of CD86 and CD163 in monocytes/macrophages and THCs. Cells were divided into four categories based on the median expression of CD86 and CD163. Right : proportion of the four categories in THCs and monocytes/macrophages. g , h The expression levels of epithelial ( g ) or mesenchymal ( h ) markers of putative THCs in the four categories. i , j Ratios of THC numbers to CTC numbers in individual patients. BCR biochemical recurrence, CR/MET castration resistance and/or metastasis. P -values were determined using a one-way ANOVA followed by Tukey test ( d , e , g , h ) and two-sided unpaired t -test ( i , j ). Source data for i , j are provided as a Source Data file.

Article Snippet: Human peripheral blood CD14 + monocytes (STEMCELL Technologies, 70035) and human peripheral blood mononuclear cells (PBMCs) (ATCC, PCS-800-081) were purchased commercially and certified by the manufacturer.

Techniques: Immunofluorescence, Derivative Assay, Expressing